diff --git a/src/components/htmlMediaHelper.js b/src/components/htmlMediaHelper.js
index fa1b343caa..21cb84e41e 100644
--- a/src/components/htmlMediaHelper.js
+++ b/src/components/htmlMediaHelper.js
@@ -28,6 +28,16 @@ function canPlayNativeHls() {
             || media.canPlayType('application/vnd.apple.mpegURL').replace(/no/, ''));
 }
 
+export function enableHlsJsPlayerForCodecs(mediaSource, mediaType) {
+    // Workaround for VP9 HLS support on desktop Safari
+    // Force using HLS.js because desktop Safari's native HLS player does not play VP9 over HLS
+    // browser.osx will return true on iPad, cannot use
+    if (!browser.iOS && browser.safari && mediaSource.MediaStreams.some(x => x.Codec === 'vp9')) {
+        return true
+    }
+    return enableHlsJsPlayer(mediaSource.RunTimeTicks, mediaType)
+}
+
 export function enableHlsJsPlayer(runTimeTicks, mediaType) {
     if (window.MediaSource == null) {
         return false;
diff --git a/src/plugins/htmlVideoPlayer/plugin.js b/src/plugins/htmlVideoPlayer/plugin.js
index 5c536e7e11..a0738ee11d 100644
--- a/src/plugins/htmlVideoPlayer/plugin.js
+++ b/src/plugins/htmlVideoPlayer/plugin.js
@@ -13,6 +13,7 @@ import {
     destroyCastPlayer,
     getCrossOriginValue,
     enableHlsJsPlayer,
+    enableHlsJsPlayerForCodecs,
     applySrc,
     resetSrc,
     playWithPromise,
@@ -515,7 +516,7 @@ export class HtmlVideoPlayer {
             elem.crossOrigin = crossOrigin;
         }
 
-        if (enableHlsJsPlayer(options.mediaSource.RunTimeTicks, 'Video') && isHls(options.mediaSource)) {
+        if (enableHlsJsPlayerForCodecs(options.mediaSource, 'Video') && isHls(options.mediaSource)) {
             return this.setSrcWithHlsJs(elem, options, val);
         } else if (options.playMethod !== 'Transcode' && options.mediaSource.Container?.toUpperCase() === 'FLV') {
             return this.setSrcWithFlvJs(elem, options, val);
diff --git a/src/scripts/browserDeviceProfile.js b/src/scripts/browserDeviceProfile.js
index 4bda92547d..3d9d08a777 100644
--- a/src/scripts/browserDeviceProfile.js
+++ b/src/scripts/browserDeviceProfile.js
@@ -451,6 +451,7 @@ export default function (options) {
 
     const canPlayVp8 = videoTestElement.canPlayType('video/webm; codecs="vp8"').replace(/no/, '');
     const canPlayVp9 = videoTestElement.canPlayType('video/webm; codecs="vp9"').replace(/no/, '');
+    const safariSupportsOpus = browser.safari && !!document.createElement('audio').canPlayType('audio/x-caf; codecs="opus"').replace(/no/, '');
     const webmAudioCodecs = ['vorbis'];
 
     const canPlayMkv = testCanPlayMkv(videoTestElement);
@@ -580,7 +581,13 @@ export default function (options) {
         if (browser.tizen) {
             hlsInTsVideoAudioCodecs.push('opus');
         }
-        if (!browser.safari) {
+        hlsInFmp4VideoAudioCodecs.push('opus');
+    }
+
+    if (browser.safari) {
+        if (safariSupportsOpus) {
+            videoAudioCodecs.push('opus');
+            webmAudioCodecs.push('opus');
             hlsInFmp4VideoAudioCodecs.push('opus');
         }
     }
@@ -655,7 +662,16 @@ export default function (options) {
     }
 
     if (canPlayVp9) {
-        mp4VideoCodecs.push('vp9');
+        if (!browser.iOS) {
+            // iOS safari may fail to direct play vp9 in mp4 container
+            mp4VideoCodecs.push('vp9');
+        }
+        // Only iOS Safari's native HLS player understands vp9 in fmp4
+        // This should be used in conjunction with forcing
+        // using HLS.js for VP9 remuxing on desktop Safari.
+        if (browser.safari) {
+            hlsInFmp4VideoCodecs.push('vp9');
+        }
         // webm support is unreliable on safari 17
         if (!browser.safari
              || (browser.safari && browser.versionMajor >= 15 && browser.versionMajor < 17)) {
@@ -672,7 +688,7 @@ export default function (options) {
         }
     }
 
-    if (canPlayVp8 || browser.tizen) {
+    if ((!browser.safari && canPlayVp8) || browser.tizen) {
         videoAudioCodecs.push('vorbis');
     }
 
@@ -744,6 +760,14 @@ export default function (options) {
         }
     });
 
+    if (safariSupportsOpus) {
+        profile.DirectPlayProfiles.push({
+            Container: 'mp4',
+            AudioCodec: 'opus',
+            Type: 'Audio'
+        });
+    }
+
     profile.TranscodingProfiles = [];
 
     const hlsBreakOnNonKeyFrames = browser.iOS || browser.osx || browser.edge || !canPlayNativeHls();